Publications of the Gehring group

Fernandez, FJ., de Vries, D., Pena-Soler, E., Coll, M., Christen, P., Gehring, H. & Vega, MC. Structure and mechanism of a cysteine sulfinate desulfinase engineered on the aspartate aminotransferase scaffold. Biochim Biophys Acta. 1824, 339-349 (2012).

Leemann-Zakaryan, R., Pahlich, S., Grossenbacher, D. & Gehring, H. Tyrosine phosphorylation in the C-terminal nuclear localization and retention signal (C-NLS) of the EWS protein. Sarcoma 2011, ID 218483 (2011).

Wu, F., Christen, P. & Gehring, H. A novel approach to inhibit intracellular vitamin B6-dependent enzymes: proof of principle with human and plasmodium ornithine decarboxylase and human histidine decarboxylase. FASEB J. 25, 2109-2122 (2011).

Müller, IB., Wu, F., Bergmann, B., Knöckel, J., Walter, RD., Gehring, H. & Wrenger, C. Poisoning pyridoxal 5-phosphate-dependent enzymes: a new strategy to target the malaria parasite plasmodium falciparum. PLoS ONE. 4(2):e4406 (2009).

Leemann-Zakaryan, R., Pahlich, S., Sedda, M., Quero, L., Grossenbacher, D. & Gehring, H. Dynamic subcellular localization of Ewing Sarcoma proto-oncoprotein and its association with and stabilization of microtubules. J. Mol. Biol. 386, 1-13 (2009).

Pahlich, S., Quero, L., Roschitzki, B., Leemann-Zakaryan, RP. & Gehring, H. Analysis of Ewing Sarcoma (EWS)-binding proteins: interaction with hnRNP M, U and RNA-helicases p68/72 with protein-RNA complexes. J. Proteome Res. 8, 4455-4465 (2009).  

Wu, F. & Gehring, H. Structural requirements for novel coenzyme-substrate derivatives to inhibit intracellular ornithine decarboxylase and cell proliferation.  FASEB J. 23, 565-574 (2009).

Curioni-Fontecedro, A., Knights, AJ., Tinguely, M., Nuber, N., Schneider, C., Thomson, CW., von Boehmer, L., Bossart, W., Pahlich, S.,  Gehring, H., Moch, H., Renner, C., Knuth, A.  & Zippelius, A. MAGE -C1/CT7 is the dominant cancer-testis antigen targeted by humoral immune responses in patients with multiple myeloma. Leukemia 22, 1646-1648 (2008).

Pahlich, S. Zakaryan, RP. & Gehring, H. Identification of proteins interacting with protein arginine methyltransferase 8: The Ewing sarcoma (EWS) protein binds independent of its methylation state. Proteins 72, 1125-1137 (2008).

Wu, F., Yu, J. & Gehring, H. Inhibitory and structural studies of novel coenzyme-substrate analogs of human histidine decarboxylase. FASEB J.  22, 890-897 (2008).

Wu, F., Grossenbacher, D. & Gehring, H. New transition state–based inhibitor for human ornithine decarboxylase inhibits growth of tumor cells. Mol. Cancer Ther. 6, 1–9 (2007).

Pahlich, S., Zakaryan, R.P. & Gehring, H. (2006) Protein arginine methylation: Cellular functions and methods of analysis, Biochimica et Biophysica Acta 1764, 1890-1903 (2006). 

Zakaryan, R.P. & Gehring, H. Identification and Characterization of the Nuclear Localization/Retention Signal in the EWS Proto-oncoprotein. J. Mol. Biol. 363, 27-38 (2006).

Pahlich, S., Bschir, K., Chiavi, C., Belyanskaya, L. & Gehring, H. Different methylation characteristics of protein arginine methyltransferase 1 and 3 toward the Ewing sarcoma protein and a peptide. Proteins-Structure Function and Bioinformatics 61, 164-175 (2005).

Fernandez, F. J., Vega, M. C., Lehmann, F., Sandmeier, E., Gehring, H., Christen, P. & Wilmanns, M. Structural studies of the catalytic reaction pathway of a hyperthermophilic histidinol-phosphate aminotransferase. Journal of Biological Chemistry 279, 21478-21488 (2004).

Belyanskaya, L. L., Delattre, O. & Gehring, H. Expression and subcellular localization of Ewing sarcoma (EWS) protein is affected by the methylation process. Experimental Cell Research 288, 374-381 (2003).

Gygi, D., Zumstein, P., Grossenbacher, D., Altwegg, L., Luscher, T. F. & Gehring, H. Human connective tissue growth factor expressed in Escherichia coli is a non-mitogenic inhibitor of apoptosis. Biochemical and Biophysical Research Communications 311, 685-690 (2003).

Minder, D., Boni, J., Schupbach, J. & Gehring, H. Immunophilins and HIV-1 infection. Archives of Virology 147, 1531-1542 (2002).

Belyanskaya, L. L., Gehrig, P. M. & Gehring, H. Exposure on cell surface and extensive arginine methylation of ewing sarcoma (EWS) protein. Journal of Biological Chemistry 276, 18681-18687 (2001).

Kasper, P., Christen, P. & Gehring, H. Empirical calculation of the relative free energies of peptide binding to the molecular chaperone DnaK. Proteins-Structure Function and Genetics 40, 185-192 (2000).

Endrich, M. M., Gehrig, P. & Gehring, H. Maturation-induced conformational changes of HIV-1 capsid protein and identification of two high affinity sites for cyclophilins in the C-terminal domain. Journal of Biological Chemistry 274, 5326-5332 (1999).

Graber, R., Kasper, P., Malashkevich, V. N., Strop, P., Gehring, H., Jansonius, J. N. & Christen, P. Conversion of aspartate aminotransferase into an L-aspartate beta-decarboxylase by a triple active-site mutation. J. Biol. Chem. 274, 31203-31208 (1999).    
    
Mouratou, B., Kasper, P., Gehring, H. & Christen, P. Conversion of tyrosine phenol-lyase to dicarboxylic amino acid beta-lyase, an enzyme not found in nature. J. Biol. Chem. 274, 1320-1325 (1999).    
    
Endrich, M. M. & Gehring, H. The V3 loop of human immunodeficiency virus type-1 envelope protein is a high-affinity ligand for immunophilins present in human blood. Eur. J. Biochem. 252, 441-446 (1998).    
    
Christen, P., Kasper, P., Gehring, H. & Sterk, M. Stereochemical constraint in the evolution of pyridoxal-5'-phosphate-dependent enzymes. A hypothesis. FEBS Lett. 389, 12-14 (1996).    
    
Endrich, M. M., Grossenbacher, D., Geistlich, A. & Gehring, H. Apoptosis-induced concomitant release of cytosolic proteins and factors which prevent cell death. Biol. Cell 88, 15-22 (1996).    
    
Kasper, P., Sterk, M., Christen, P. & Gehring, H. Molecular-dynamics simulation of domain movements in aspartate aminotransferase. Eur. J. Biochem. 240, 751-755 (1996).    
    
Graber, R., Kasper, P., Malashkevich, V. N., Sandmeier, E., Berger, P., Gehring, H., Jansonius, J. N. & Christen, P. Changing the Reaction Specificity of a Pyridoxal-5'-Phosphate-Dependent Enzyme. Eur. J. Biochem. 232, 686-690 (1995).    
    
Malashkevich, V. N., Jager, J., Ziak, M., Sauder, U., Gehring, H., Christen, P. & Jansonius, J. N. Structural Basis for the Catalytic Activity of Aspartate-Aminotransferase K258h Lacking the Pyridoxal 5'-Phosphate-Binding Lysine Residue. Biochemistry 34, 405-414 (1995).    
    
Pan, P., Jakob, C. A., Sandmeier, E., Christen, P. & Gehring, H. Modulation of the Activity of Mitochondrial Aspartate-Aminotransferase H352c by the Redox State of the Engineered Interdomain Disulfide Bond. J. Biol. Chem. 269, 25432-25436 (1994).    
    
Pan, P., Jaussi, R., Gehring, H., Giannattasio, S. & Christen, P. Shift in Ph-Rate Profile and Enhanced Discrimination between Dicarboxylic and Aromatic Substrates in Mitochondrial Aspartate-Aminotransferase Y70h. Biochemistry 33, 2757-2760 (1994).    
    
Schmid, D., Baici, A., Gehring, H. & Christen, P. Kinetics of Molecular Chaperone Action. Science 263, 971-973 (1994).    
    
Sterk, M., Hauser, H., Marsh, D. & Gehring, H. Probing Conformational States of Spin-Labeled Aspartate-Aminotransferase by Esr. Eur. J. Biochem. 219, 993-1000 (1994)

Geistlich, A. & Gehring, H. Cdgf (Chicken-Embryo Fibroblast-Derived Growth-Factor) Is Mitogenically Related to Tgf-Beta and Modulates Pdgf, Bfgf, and Igf-I Action on Sparse Nih/3t3 Cells. Exp. Cell Res. 204, 329-335 (1993).

Hartmann, C. M., Gehring, H. & Christen, P. The Mature Form of Imported Mitochondrial Proteins Undergoes Conformational-Changes Upon Binding to Isolated-Mitochondria. Eur. J. Biochem. 218, 905-910 (1993).

Ziak, M., Jager, J., Malashkevich, V. N., Gehring, H., Jaussi, R., Jansonius, J. N. & Christen, P. Mutant Aspartate-Aminotransferase (K258h) without Pyridoxal-5'-Phosphate-Binding Lysine Residue - Structural and Catalytic Properties. Eur. J. Biochem. 211, 475-484 (1993).